A Double Infiltration Technic for Serial Sectioning of Heavily Sclerotized Insects
作者:
TrombettaL. D.,
ForbesJ.,
期刊:
Journal of Histotechnology
(Taylor Available online 1977)
卷期:
Volume 1,
issue 1
页码: 25-26
ISSN:0147-8885
年代: 1977
DOI:10.1179/his.1977.1.1.25
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractA double infiltration method using n-butyl alcohol in conjunction with celloidin and paraplast plus (BCPP) is described. Serial sections cut at 4 microns thick of heavily sclerotized insect cuticle fixed with alcoholic Bouin's fluid are easily obtained. The architecture of the cuticle and the morphology of the underlying tissue show little distortion and excellent staining.
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