Some remarks on the accuracy of surface area estimation using the spatial grid
作者:
K. SANDAU,
U. HAHN,
期刊:
Journal of Microscopy
(WILEY Available online 1994)
卷期:
Volume 173,
issue 1
页码: 67-72
ISSN:0022-2720
年代: 1994
DOI:10.1111/j.1365-2818.1994.tb03428.x
出版商: Blackwell Publishing Ltd
关键词: Counting rule;covariogram;spatial grid;stereology;surface area estimation;variance estimation
数据来源: WILEY
摘要:
SUMMARYA set of three line grids in three orthogonal directions is called a spatial grid. This spatial grid can be used for surface area estimation by counting the number of intersection points of a surface with the grid lines. If direction and localization of the spatial grid are suitably randomized, the expectation of this number is proportional to the surface area of interest. The method was especially developed for cases where the surface to be measured is embedded in a medium, which is the usual case in microscopical applications, and where a stack of serial optical sections of the surface is available.The paper presents an improvement of an earlier version of the counting rule for intersection points. Furthermore, if the direction of sectioning is not uniform random, a bias results. This bias is calculated for a disc as a perfectly anisotropic object. A generalization of the estimator is considered by introducing a weighted mean instead of the usual arithmetic mean. The variance due to the randomized direction is investigated depending on the weights, and the minimum of this variance is derived. The relationship between the covariogram and the variance of the surface area estimated with the spatial grid is considered.
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