Comparison between two methods of generating pressure—volume curves
作者:
G. A. RITCHIE,
J. R. RODEN,
期刊:
Plant, Cell&Environment
(WILEY Available online 1985)
卷期:
Volume 8,
issue 1
页码: 49-53
ISSN:0140-7791
年代: 1985
DOI:10.1111/j.1365-3040.1985.tb01208.x
出版商: Blackwell Publishing Ltd
关键词: Pseudotsuga menziesii(Mirb.) Franco;Pinaceae;Douglas fir;water potential;osmotic potential;pressure volume curve;pressure chamber
数据来源: WILEY
摘要:
Abstract.Pressure—volume (P—V) curves were generated on roots and shoots of coastal Douglas fir [Pseudotsuga menziesii(Mirb.) Franco] seedlings using two procedures. In the first (Method A), samples were dehydrated inside a pressure chamber. Exuded stem sap was collected and weighed at successive pressure increases to derive the P—V curve. In the second method (Method B). excised samples were allowed to dry outside the pressure chamber by evapotranspiration. They were weighed periodically to determine sap loss and their corresponding balance pressures were determined in a pressure chamber in order to derive the P—V curve.Estimates of volume averaged osmotic potential at full turgor and water potential at zero turgor which were derived graphically from the P—V curves, were different for each method. In general, estimates were more negative in Method A, by as much as 1.5 MPa in one case. Also, Method B did not record an osmotic adjustment in seedlings which were subjected to severe water stress while Met
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