‘Lymphocyte-defined’antigens on corneal fibroblasts
作者:
YoungElaine,
McMillanBarbara A.,
期刊:
Current Eye Research
(Taylor Available online 1986)
卷期:
Volume 5,
issue 4
页码: 273-281
ISSN:0271-3683
年代: 1986
DOI:10.3109/02713688609020053
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Human stromal (fibroblast) cultures were established from random, normal corneas obtained from the International Eye Bank in Baltimore. Cultures were assessed for the expression of Class I, Class II, monocyte/granulocyte and T-cell antigens before and after treatment with gamma or alpha interferon (IFN), interleukin 2, and 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA). Class I (B2microglobulin) and monocyte/granulocyte (Leu M-1) antigens were evident before induction, natural killer cell (Leu 7) antigens were transiently expressed, and Class II (HLA-DR, Leu 10) antigens were not expressed until after induction with gamma IFN. PMA reduced the numbers of cells positive for Leu M-1 while alpha IFN and interleukin 2 had no effect on antigen expression. Kinetic studies of HLA-DR induction indicated that as little as 12 hours exposure to gamma IFN was sufficient to initiate induction and that 30% of the population was still expressing HLA-DR 7 days after removal of gamma IFN. The inducible expression of Class II antigens following lymphokine exposure may play a critical role in rejection of allografted corneas.
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