Two abnormal hemoglobins were accidentally detected by cation exchange high performance liquid chromatography with the Diamat system of Bio-Rad Laboratories; the variants eluted together with the fast-moving Hb A1c. Structural analysis of isolatedβchains and sequence analysis of amplified DNA identified a new variant, i.e. Hb Graz that has a His→Leu replacement at position 2 of theβchain, in four healthy, apparently unrelated, adults. The second variant was identical to Hb Sherwood Forest orα2β2104(G6)Arg→Thr; it is believed that this may be the second observation of this abnormal hemoglobin.