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Instrument to Measure Fluorescence Lifetimes in the Millimicrosecond Region

 

作者: Seymour Steven Brody,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1957)
卷期: Volume 28, issue 12  

页码: 1021-1026

 

ISSN:0034-6748

 

年代: 1957

 

DOI:10.1063/1.1715792

 

出版商: AIP

 

数据来源: AIP

 

摘要:

An instrument to measure fluorescence lifetimes of the order of millimicroseconds is described. It utilizes a hydrogen flash lamp and high‐speed oscilloscope. The fluorescence decay is displayed on the oscilloscope, and using the method of moments the observed fluorescence is corrected for the response of the lamp and the detection equipment. The instrument was used to study the primary photochemical processes of photosynthesis. Measured lifetimes are given for the various photosynthetic pigments (in vitro), some organic dyes, and chlorophyll fluorescence in algae. With this instrument it has been possible to measure the delayed energy transfer in living systems.

 

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