An analytical method for indirect trace determination of ethylenediaminetetraacetic acid (EDTA) in water, by potentiometric stripping analysis is described. Excess Bi(III) was added to form a 1:1 complex with EDTA at pH 2.3. The uncomplexed Bi(III) was then deposited on a glassy carbon electrode at a potential of −0.40 V vs. SCE and subsequently stripped potentiometrically using potassium dichromate as oxidant. The stripping time of uncomplexed Bi(III) was recorded. The Concentration of EDTA in the sample was determined from the concentration of added Bi(III) and the potentiometrically stripped Bi(III) at −0.4 V by the standard addition method. The relative standard deviation for EDTA concentration of 95 ppb and 4.5 ppb was 1.9% and 2.6%, respectively. The detection limit was about 1 ppb EDTA for a deposition time of 3 minutes.