Neutrophil Motility in Crohn’s Disease
作者:
F.T. Curran,
M.R.B. Keighley,
期刊:
Digestion
(Karger Available online 1991)
卷期:
Volume 48,
issue 2
页码: 104-112
ISSN:0012-2823
年代: 1991
DOI:10.1159/000200680
出版商: S. Karger AG
关键词: Polymorphonuclear leucocyte;Chemotaxis;Crohn’s disease
数据来源: Karger
摘要:
Neutrophil motility in normal subjects and Crohn’s disease patients was assessed using both double and single filter assay systems with n-formylmethionylleucylphenylalanine (FMLP) as the attractant, and in a single filter assay with Candida albicans in 10% serum as the attractant. Assays were performed in triplicate and included controls to eliminate the effect of random (non-stimulated) locomotion. After incubation at 37 °C, filters were fixed and stained and the numbers of neutrophils per filter field counted under the microscope. Stimulated migration was calculated by subtracting random from total migration. Compared to normal neutrophils, 56% more Crohn’s disease neutrophils migrated towards FMLP in the double filter system (p = 0.01). With the single filter technique using FMLP as the attractant 58% more Crohn’s disease neutrophils migrated (p = 0.002). With the single filter technique using C. albicans as the attractant 47% more Crohn’s disease neutrophils migrated (p = 0.02). Neutrophil motility in patients with Crohn’s disease is enhanced and results were not related to diseas
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