A Comparison of the Molecular Dynamics of Fluorescent Probes in Curved Lipid Vesicles and Planar Multibilayers
作者:
HermanVan Langen,
GijsVan Ginkel,
YehudiK. Levine,
期刊:
Liquid Crystals
(Taylor Available online 1988)
卷期:
Volume 3,
issue 10
页码: 1301-1317
ISSN:0267-8292
年代: 1988
DOI:10.1080/02678298808086612
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The dynamic behaviour of the probe molecules DPH and TMA-DPH embedded in small unilamellar vesicles and planar multibilayers of POPC has been studied by time-resolved fluorescence depolarization techniques. The molecular dynamics of the probe molecules was analysed in terms of the rotational diffusion model. It is found that analysis of the time-dependent fluorescence anisotropy from the vesicle system yields two distinct, though statistically equivalent solutions. On the other hand the measurements on planar multibilayers can be interpreted unequivocally. It is shown that the order parameters of the probe molecules are higher in the multibilayers than in the vesicles. A reconstruction of the orientational distribution function reveals that the TMA-DPH molecules have a more pronounced tendency to lie with their long axes parallel to the bilayer surface in the curved vesicles than in the planar multibilayers. An intriguing finding is that the reorientational motion of the probes is considerably slower in the multibilayer samples than in the vesicles. These differences are attributed to the curvature and higher hydration of the bilayers in the vesicle systems.
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