Survival of structure and function in postmortem rat and human retinas: rhodopsin regeneration, cGMP and the ERG
作者:
HuangJun C.,
VoadenMary J.,
MarshallJohn,
期刊:
Current Eye Research
(Taylor Available online 1990)
卷期:
Volume 9,
issue 2
页码: 151-162
ISSN:0271-3683
年代: 1990
DOI:10.3109/02713689008995201
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Procedures for regenerating visual pigment and restoring phototransduction have been established with freshly isolated, bleached rat retinas. Phosphatidyl choline liposomes containing a 500μM mixture of retinal isomers, including the 9-cis and 11-cis forms, were employed and the results compared with dark-adapted retinas, incubated similarly but without retinal. The following were recovered in a 60 min incubation, rhodopsin (plus isorhodopsin) to 91% of the original rhodopsin concentration, 87% of cGMP and 89% of PHI amplitude at saturation. PIII amplitude vs. log intensity curves gave values of n between 0.6 and l.o andσbetween 85 and 439 quanta/μm2.Human retinas, ranging from 18 to 58 hours postmortem and treated as above, also produced photoresponses. Of the 7 retinas studied so far, rhodopsin has been regenerated to 0.1-0.35 nmol/mg protein, cGMP to 23.5-49.2 pmol/mg protein, and PIII to 20-50μV: in some cases a b-wave was also seen. Values of n varied between 0.6 and 1.0, andσbetween 132 and 3700 quanta/μm2. PIII responses were also seen after retinas, approximately 30 hours postmortem, were incubated for a further 24 hours in fortified medium. After incubation, retinal vacuolation was reduced.
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