Binding of 5-Methoxypsoralen to Blood Fractions
作者:
Patrice Muret,
Safwat Makki,
Saik Urien,
Pierre Agache,
Pierre Bechtel,
Jean-Paul Tillement,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1993)
卷期:
Volume 6,
issue 1
页码: 45-51
ISSN:1660-5527
年代: 1993
DOI:10.1159/000211084
出版商: S. Karger AG
关键词: 5-Methoxypsoralen;Binding;Blood fractions;Human serum albumin;Free fatty acids;PUVA therapy
数据来源: Karger
摘要:
The binding of 5-methoxypsoralen (5-MOP) to human serum and blood fractions was studied by equilibrium dialysis associated to high-performance liquid chromatography. 5-MOP serum binding was 95% and kept constant in the range of therapeutic concentrations. Albumin was the main binding protein with one class of binding sites (n = 2.05) and with a moderate affinity constant (Ka = 10,270 M-1). Free fatty acids (FFA) enhanced 5-MOP binding to albumin. Binding to other proteins, α1-acid glycoprotein, gamma-globulins and lipoproteins (LDL, HDL, VLDL) also occurred, but was negligible. 5-MOP bound to red blood cells (RBCs) with a binding index (ratio of intra- to extra-globular 5-MOP concentrations) of 3.84 and 1.48 in buffer and plasma, respectively. 5-MOP distribution in blood was simulated. The free fraction of 5-MOP in blood could be increased in hypoalbuminemia, unchanged in acute inflammation, and decreased by FFA
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