Nuclear binding sites for juvenile hormone III in the male accessory reproductive glandsof Melanoplus sanguinipes
作者:
SHEIKHM. ISMAIL,
CEDRIC GILLOTT,
期刊:
Invertebrate Reproduction & Development
(Taylor Available online 1997)
卷期:
Volume 31,
issue 1-3
页码: 257-262
ISSN:0792-4259
年代: 1997
DOI:10.1080/07924259.1997.9672584
出版商: Taylor & Francis Group
关键词: Juvenile hormone;Melanoplus sanguinipes;male accessory reproductive gland;nuclear binding sites
数据来源: Taylor
摘要:
We have identified a potential nuclear juvenile hormone (JH) receptor in the long hyaline tubules (LHT), part of the male accessory reproductive gland (MARG) ofM. sanguinipes.The MARG was incubatedin vitrowith [3H]JH III, and the distribution of the [3H]JH III among the cellular fractions of the LHT was determined. Some 37±4% of the radioactivity was associated with the crude nuclear pellet, while the cytosolic, microsomal and mitochondrial fractions contained 30±3%, 23±2% and 10±1%, respectively. The bound JH III was measured in nuclear extracts of LHT from males up to 15 days post-eclosion. These results revealed that JH binding increased in an age-dependent manner up to day 7, then levelled off to day 12, to increase again on day 14. The nuclear-binding component in the LHT had a very strong affinity for JH III, with a KDvalue of 0.8 nM. Our observations are considered in relation to the potential site and mode of action of JH.
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