Specific Antibody Responses to Synthetic Peptides of HIV‐1 p17 Correlate with Different Stages of HIV‐1 Infection
作者:
Jian-Dong Jiang,
Fo-Nian Chu,
Paul Naylor,
Janet Kirkley,
John Mandeli,
Joyce Wallace,
Prem Sarin,
Allan Goldstein,
James Holland,
J. Bekesi,
期刊:
Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 1992)
卷期:
Volume 5,
issue 4
页码: 382-390
ISSN:0894-9255
年代: 1992
出版商: OVID
关键词: Synthetic epitope;HIV-1;Clinical status;p17;Synthetic peptides
数据来源: OVID
摘要:
Antibodies were determined against five synthetic peptides (epitopes) of HIV-1 p17 in the sera of an immunologically and clinically well-characterized cohort (N = 292) of HIV-1 seronegative and HIV-1 seropositive high-risk homosexual men, HIV-1 seropositive i.v. drug abusers (IVDA), and AIDS patients. The synthetic peptides, representing the entire HIV-1 p17 protein sequence were: HGP-33 (aa 1–33), HGP-19 (aa 34–52), HGP-35 (aa 51–85), HGP-30 (aa 85–114), and HGP-17 ala (aa 114–131). The presence of one or more peptide-specific antibodies in the sera of all of the HIV-1 p17-positive subjects indicated that all five peptides contain B-cell epitopes. No antibodies were found in the sera of heterosexual controls, HIV-1 seronegative high-risk men, or asymptomatic HIV-1 seropositive but p17 antibody-negative study subjects. Significant differences in antibody recognition profiles to the peptide epitopes were found among the various study groups. A significantly higher proportion of HIV-1 seropositive IVDA had antibodies specific to HGP-17 ala (aa 114–131), HGP-35 (aa 51–85), and HGP-33 (aa 1–33) compared to the HIV-1 p17-positive asymptomatic homosexuals. The epitope-specific antibody responses reflected the clinical status of the HIV-1-infected study subjects, and declined to nondetectable levels as the patient progressed to ARC/AIDS. This decline preceded by several months the reduction in the antibody titer against the intact HIV-1 p17 and p24 proteins. Thus, the antibody response against specific HIV-1 p17 epitopes might be a better prognostic indicator than the total antibody response against the native HIV-1 p17 protein.
点击下载:
PDF
(634KB)
返 回