31P NUCLEAR MAGNETIC RESONANCE OF RAT PANCREATIC GRAFTS1
作者:
GARETH MORRIS,
STEPHEN WILLIAMS,
EDWARD PROCTOR,
DAVID GADIAN,
NORMAN BROWSE,
期刊:
Transplantation
(OVID Available online 1989)
卷期:
Volume 47,
issue 5
页码: 779-783
ISSN:0041-1337
年代: 1989
出版商: OVID
数据来源: OVID
摘要:
This study investigates whether phosphate metabolite concentrations and intracellular pH alter in early acute rejection of rat pancreatic allografts. In vitro biochemical assays, in vitro31P nuclear magnetic resonance spectroscopy, and in vivo31P NMR spectroscopy of the grafts were compared. Duct-ligated, vascularized rat pancreatic isografts and allografts were transplanted onto the infrarenal aorta of the recipients with inferior vena cava venous drainage. In order to obtain reproducible acute rejection, allografting was performed across a major histocompatibility barrier. For the in vitro experiments freeze-clamped graft extracts were prepared and analyzed for adenosine triphosphate concentration by fluorimetry, then placed in an 8.5 Tesla vertical bore magnet.31P NMR spectra were recorded using a Bruker AM 360 spectrometer operating at 145.7 MHz for31P. Spectra were acquired from nontransplanted controls; 3-day, 5-day, and 1-month posttransplant isografts, and 3-day and 5-day posttransplant allografts. All grafts examined were functioning satisfactorily. The ATP content of the extracts was significantly lower in the 3− and 5-day allografts than the respective isografts. Invasive in vivo31P NMR spectra were recorded using surface coils adjacent to the grafts from functioning 5-day posttransplant isografts and allografts (i.e., 3 days prior to an expected elevation in blood sugar from acute rejection in the allografts). The ATP/inorganic phosphate ratios and pH from the in vivo spectra were significantly lower in the allografts than in the isografts. It is concluded that changes in intracellular metabolism occur early in the process of acute rejection and that31P NMR spectroscopy may provide a means of diagnosing this before current methods.
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