DNA damage, Repair, and Replication in Selenite-Induced Cataract in Rat Lens
作者:
LiLi,
HessJohn L.,
BunceG. E.,
期刊:
Current Eye Research
(Taylor Available online 1990)
卷期:
Volume 9,
issue 11
页码: 1041-1050
ISSN:0271-3683
年代: 1990
DOI:10.3109/02713689008997578
出版商: Taylor&Francis
数据来源: Taylor
摘要:
DNA synthesis was evaluated in vitro by measuring incorporation of3H-thymidine in rat lens following systemic delivery of a cataractogenic dose of selenite. Among early metabolic changes observed in the lenses of rats receiving a single dose of 30 nmol Na2SeO3/g body weight was a 30% decrease in DNA replication in lens epithelium occurring between 6 and 12 h after administration of the selenite. This change was followed by an 80% increase in replication by 24 h. Thymidine incorporation in DNA remained elevated compared to controls through 96 h. Unscheduled DNA synthesis was found to be approximately 10% of the total DNA formed, but there was a 30% and 70% increase of this putative DNA repair in the lenses from selenite-treated animals at 6 and 24 h after the injection. Using the alkaline unwinding assay, the proportion of single-strand DNA in lenses from selenite-treated animals increased after 24 h. This estimate of DNA damage was greater in lenses after 96 h. Each component of DNA metabolism: damage, repair, and replication, was affected by the occurrence of selenite stress in lens. These changes both preceded and accompanied nuclear cataract formation.
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