A Versatile Fluorogenic Labelling Reagent for Primary and Secondary Amines: 9-Fluorenylmethyl Chloroformate
作者:
H.Anson Moye,
A.J. Boning,
期刊:
Analytical Letters
(Taylor Available online 1979)
卷期:
Volume 12,
issue 1
页码: 25-35
ISSN:0003-2719
年代: 1979
DOI:10.1080/00032717908082516
出版商: Taylor & Francis Group
关键词: Amines;liquid chromatography;fluorogenic;lablelling;9-fluorenylmethyl chloroformate
数据来源: Taylor
摘要:
Both primary and secondary amines may be readily labelled with the 9-fluorenylmethoxy carbonyl group by the reaction with 9-fluorenylmethyl chloroformate (FMOCCI). The reaction proceeds rapidly under alkaline aqueous conditions, allows for simple elimination of excess reagent by partitioning, improves chroatographic resolution of amino acid-like compounds by anion exchange liquid chromatography and introduces a fluorophore with a large quantum yield which is essentially unaffected by solvent-solute interactions.
点击下载:
PDF (290KB)
返 回