Detection of point mutations in the p53 gene: Comparison of single‐strand conformation polymorphism, constant denaturant gel electrophoresis, and hydroxylamine and osmium tetroxide techniques
作者:
Alison Condie,
Rosalind Eeles,
Anne‐Lise Borresen,
Christopher Coles,
Colin Cooper,
Jane Prosser,
期刊:
Human Mutation
(WILEY Available online 1993)
卷期:
Volume 2,
issue 1
页码: 58-66
ISSN:1059-7794
年代: 1993
DOI:10.1002/humu.1380020111
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Point mutation;p53;SSCP;CDGE;HOT;Chemical mismatch
数据来源: WILEY
摘要:
AbstractA comparison was made between the 3 most commonly used techniques for the detection of point mutations: single‐strand conformation polymorphism (SSCP), constant denaturant gel electrophoresis (CDGE), and hydroxylamine and osmium tetroxide used in amplification mismatch cleavage analysis (HOT). Using human DNA samples containing known mutations in the p53 gene, SSCP detected 90% of mutations (18/20), CDGE detected 88% (15/17) pre‐decoding of the samples but 100% when the mutations were known and the CDGE conditions optimized, and the HOT technique was 100% accurate, although 1 mutation was missed through careless examination of the gel. The positive and negative aspects of each of the techniques are considered and suggestions are made regarding the particular situations in which each of them is most useful. © 1993 Wiley‐Lis
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