Giving the rear wheels of manually propelled wheelchairs negative camber (by angling them such that the bottoms of the wheels are farther apart than the tops) has become increasingly popular, especially among active users. This study's purpose was to evaluate how changing camber affects a wheelchair's mechanics and the extent of these changes. Using a representative wheelchair occupied by an anthropomorphic dummy, we altered the camber from +15° to −15° and measured the effects on nine selected variables. In addition to increasing the wheelchair's track width, negative camber increases the wheelchair's wheelbase and decreases the height of the frame. With negative camber, the center of gravity (CG) of the occupied wheelchair moves backwards. Negative camber causes a backwards tilt of the frame which, in turn, causes toe-out of the rear wheels, a positive caster-inclination angle and an increase in the caster-trail distance. It is apparent that a change in one component of a wheelchair can affect many others, which should be taken into consideration and, where appropriate, compensated for when changing the camber of the wheelchair.