RAPD analysis of selected common bean (Phaseolus vulgarisL.) cultivars from three African countries
作者:
C.M.S. Mienie,
L. Herselman,
R.E. Terhlanche,
期刊:
South African Journal of Plant and Soil
(Taylor Available online 2000)
卷期:
Volume 17,
issue 2
页码: 93-94
ISSN:0257-1862
年代: 2000
DOI:10.1080/02571862.2000.10634876
出版商: Taylor & Francis Group
关键词: RAPD;dry bean cultivars
数据来源: Taylor
摘要:
DNA from six dry bean cultivars was analysed with a total of thirty two primers in RAPD analysis to develop a method to distinguish between the different cultivars. The varieties are cultivated in three African countries and belong to two different gene pools. The cultivars analysed were Lyamunga 85 and Lyamunga 90 from Tanzania, CAL 96 and K 20 from Uganda, and Awash I and Mexican 142 from Ethiopia. The two types of beans, the Mesoamerican white seeded and large seeded Andean calima types, were separated in the two genetic pools. RAPD analysis revealed a close relationship between Lyamunga 85 and CAL 96. All the cultivars tested could be distinguished with a minimum of six primers.
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