Two Methods for Flat Embedding Sections in LR White Cut from Paraffin Blocks
作者:
MigheliAntonio,
AttanasioAngelo,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 2
页码: 89-92
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110557
出版商: Taylor&Francis
关键词: flat embedding;LR White;immuno-gold staining
数据来源: Taylor
摘要:
Two simple methods are described for flat embedding of sections cut from paraffin blocks of brain tissue in the hydrophilic resin LR White. The ability of fresh resin to polymerize when added to already cured resin is exploited. In the first method, a tissue section with a drop of LR White is pressed to the bottom of a gelatin capsule using a blank block. In the second method, the section in a drop of resin is sandwiched between the sawed halves of a blank block. After curing, thin sections containing large areas of tissue can be collected and processed for immunocytochemistr y.
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