Modified Tetrachrome Method for Osteoid and Defectively Mineralized Bone in Paraffin Sections
作者:
RalisZ. A.,
WatkinsG.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 6
页码: 339-345
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110046
出版商: Taylor&Francis
关键词: bone;osteoid;mineralization;osteomalacia;rickets;osteoporosis;bone tissue defects;defective mineralization
数据来源: Taylor
摘要:
A new modification of the tetrachrome method for bone osteoid in paraffin sections has been designed. The modified tetrachrome method suitable for routine use in any histology laboratory retains the simplicity of the original method and gives good results on the freshly fixed, decalcified, paraffin embedded material. Osteoid tissue is stained deep blue and normally mineralized bone is stained red. Defectively mineralized bone stains pale blue or pink and the cellular population is clearly identifiable. The ability to distinguish the osteoid tissue from mineralized bone and connective tissue and cartilage makes diagnosis of osteomalacia or osteoid producing tumors or assessment of ossification process straightforward, without the need for un-decalcified sections. By displaying simultaneously irregularities in the mineralized matrix and morphology of bone cells, the method also permits the diagnosis of conditions recently described in patients with osteoporotic fractures, such as osteocytic degeneration and bone tissue defects.
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