Molecular size and charge characterization by rheophoresis I. Theory and gel calibration
作者:
Henrik Waldmann‐Meyer,
Per Amstrup Pedersen,
期刊:
ELECTROPHORESIS
(WILEY Available online 1984)
卷期:
Volume 5,
issue 1
页码: 18-21
ISSN:0173-0835
年代: 1984
DOI:10.1002/elps.1150050103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractRheophoresis,i.e.thin‐layer electrophoresis in particulate gels with controlled evaporation, combines in one experiment the chromatographic and the electrophoretic characteristics of macromlecules in such a way that they can be determined independently of each other. In this paper theory and experimental corroboration for molecular size determination are presented. We describe the migration velocity of any molecule as the sum of an electrophoretic, an electroosmotic and a rheophoretic component. The latter is the velocity originating from the liquid flow produced by evaporation. It is a function of the position along the gel axis and the cross‐section available to the molecule, but totally independent of the molecular charge. As a result of the position dependence, the rheophoretic component can be isolated from the two others and the available cross‐section determined by means of a simple plot. Thus, the chromatographic partition coefficient is readily calculated and the effective hydrodynamic radius of the molecule obt
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