Minimal effect of brain temperature changes on glutamate release in rat following severe global brain ischemiaa dialysis electrode study
作者:
Satoshi Asai,
Heng Zhao,
Yasuo Takahashi,
Toshihito Nagata,
Tadashi Kohno,
Koichi Ishikawa,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 17
页码: 3863-3867
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Dialysis electrode;Glutamate;Ischemia;Mild hyperthermia;Mild hypothermia;Normothermia;Rat;Release;Re-uptake;Reversed uptake
数据来源: OVID
摘要:
USING a dialysis electrode, we recently developed an oxygen-independent system for real-time measurement of the glutamate concentration in the extracellular space ([Glu]e) during ischemia. This system allows separate evaluation of intra-ischemic biphase [Glu]e elevation, i. e. release from synaptic vesicles (1st phase), reversed uptake of glutamate from metabolic pools in neuronal cells (2nd phase), and post-ischemic glutamate re-uptake in ischemia—reperfusion models. Using the system, we attempted to clarify the relationship between biphase glutamate release and brain temperature in a model of acute global ischemia produced by transecting both carotid arteries. Our results showed that, in contrast to mild hyperthermia, hypothermia did not inhibit the 1st phase of [Glu]e release, and changes in intra-ischemic brain temperature had a minimal effect on the 2nd phase of [Glu]e elevation during severe acute ischemia. These findings, together with our previous data, indicate that brain temperature change in the intra-ischemic period plays an important role in disturbance of the glutamate re-uptake system during ischemia.NeuroReport9: 3863–3868 © 1998 Lippincott Williams & Wilkins.
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