Restricted Heterogeneity and Changing Spectrotypes in Autoantibodies to La/SS-B
作者:
SatohMinoru,
AkizukiMasashi,
YamagataHajime,
NakayamaShoji,
HommaMitsuo,
期刊:
Autoimmunity
(Taylor Available online 1996)
卷期:
Volume 24,
issue 4
页码: 229-236
ISSN:0891-6934
年代: 1996
DOI:10.3109/08916939608994715
出版商: Taylor&Francis
关键词: anti-La antibodies;antinuclear antibodies;isoelectric focusing;spectrotype
数据来源: Taylor
摘要:
Isoelectric focusing (IEF) spectrotype of specific immunoglobulins has been studied as a marker for B-cell clonality. In the present study, the spectrotype of anti-La antibodies in human autoimmune sera were analyzed by newly developed IEF sandwich assay in which focused total immunoglobulin on filter papers are incubated with crude antigen followed by horse-radish peroxidase-labeled anti-La antibodies. The anti-La spectrotypes contained oligoclonal bands, the positions and patterns of which are different in each patient, suggesting the preferential expansion of limited numbers of anti-La producing B-cell clones unique to individual patients. Furthermore, the bands on anti-La spectrotype in sequentially obtained sera changed continuously, suggesting alteration in the expanding anti-La producing clones. These may reflect affinity maturation and/or diversification of the B-cell epitopes involving somatic mutation.
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