In Vitro Combined Effects of a Triazene Compound and Interferon-Beta on Natural Immunity Against Lymphoblastoid Cells: Studies at Effector and Target Cell Level
作者:
BonmassarL.,
D'atriS.,
TurrizianiM.,
GiulianiA.,
期刊:
Immunopharmacology and Immunotoxicology
(Taylor Available online 1994)
卷期:
Volume 16,
issue 4
页码: 695-715
ISSN:0892-3973
年代: 1994
DOI:10.3109/08923979409019746
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractIt was shown that Dacarbazine and other triazene compounds render murine leukemias highly immunogenic and susceptible to natural immunity (NI). In addition a pilot clinical study revealed that Dacarbazine can be cytotoxic for bone marrow blasts in patients with acute non-lymphoblastic leukemias through a mechanism that could be, at least in part, of immunological origin. However triazenes depress antigen-dependent responses and NI, whereas interferons, including interferon-beta (IFN), antagonize drug-induced impairment of NI. Therefore the complex interaction between triazenes and IFN on NI effector (i.e. NK) lymphocytes and human target lymphoblastoid cells has been investigated. the results show that: (a) EFN increases NK activity and antagonizes the depressive effects of methyl-triazene-benzoic acid (MTBA, an in vitro active triazene compound) on the NK function; (b) a lymphoblastoid cell line exposed to multiple in vitro treatments with MTBA, shows increased growth rate, augmented chemoresistance to MTBA, and higher susceptibility to NI than parental cells; (c) as expected IFN pretreatment down-regulates the susceptibility of lymphoblastoid cells to NK effectors; (d) however a net“therapeutic gain”was found if the overall influence of MTBA + IFN on target and effector cells is considered.
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