Monoclonal Antibodies Specific for Sickle Cell Hemoglobin
作者:
JensenR. H.,
VanderlaanM.,
GrabskeR. J.,
BranscombE. W.,
BigbeeW. L.,
StankerL. H.,
期刊:
Hemoglobin
(Taylor Available online 1985)
卷期:
Volume 9,
issue 4
页码: 349-362
ISSN:0363-0269
年代: 1985
DOI:10.3109/03630268508997010
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Two mouse hybridoma cell lines were isolated which produce monoclonal antibodies that bind hemoglobin S. The mice were immunized with peptide-protein conjugates to stimulate a response to the amino terminal peptide of the beta chain of hemoglobin S, where the single amino acid difference between A and S occurs. Immunocharacterization of the antibodies shows that they bind specifically to the immunogen peptide and to hemoglobin S. The specificity for S is high enough that one AS cell in a mixture with a million AA cells is labeled by antibody, and such cells can be analyzed by flow cytometry. Immunoblotting of electrophoretic gels allows definitive identification of hemoglobin S as compared with other hemoglobins with similar electrophoretic mobility.
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