Effect of Inducers on the Activity of Glutathione S-Transferase and Other Enzymes of the Glutathione Pathway in Cultured Human Keratinocytes
作者:
Donald A. Vessey,
Kyung-Hee Lee,
Kerry L. Blacker,
Thomas D. Boyer,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1993)
卷期:
Volume 6,
issue 4
页码: 241-245
ISSN:1660-5527
年代: 1993
DOI:10.1159/000211144
出版商: S. Karger AG
关键词: Keratinocytes;Glutathione S-transferases;Glutathione reductase;Glutathione peroxidase;Phenobarbital;Trans-stilbene oxide;Propylthiouracil;Butylated hydroxyanisole;Induction
数据来源: Karger
摘要:
Known inducers of the hepatic glutathione (GSH) S-transferases were tested at the limits of their solubility as inducers of the enzyme in cultured human keratinocytes. Neither phenobarbital, trans-stilbene oxide, propylthiouracil, nor butylated hydroxyanisole increased GSH S-transferase activity or led to the appearance of α- or μ-forms of the enzyme, as judged by Western blotting. Only the π-form of the enzyme was found before and after all treatments. Thus, the enzyme is not indu-cible in keratinocytes. However, 4 mM propylthiouracil did lead to a 50% increase in GSH reductase activity, and phenobarbital at 4 mM completely abolished GSH peroxidase and GSH reductase activity and led to a significant loss of viabili
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