The role of arachidonic acid release and lipoxygenase pathway in lipopolysaccharide‐induced thromboplastin activity in monocytes
作者:
B. østerud,
J. Olsen,
L. Wilsgàrd,
期刊:
Blood Coagulation and Fibrinolysis
(OVID Available online 1990)
卷期:
Volume 1,
issue 1
页码: 41-46
ISSN:0957-5235
年代: 1990
出版商: OVID
关键词: Arachidonic acid metabolism;monocyte activation;platelets;thromboplastin
数据来源: OVID
摘要:
Lipopolysaccharide (LPS) stimulation of human monocytes in heparinized whole bloodin vitroas expressed by induced activity of thromboplastin, has been studied. An essential role of arachidonic acid (20:4) release was found. 2,4‘-dibromoacetophenone, a phospholipase A2inhibitor, totally blocked the induced synthesis of thromboplastin activity. Furthermore, nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, had an effect on the LPS-induced thromboplastin synthesis which varied from no inhibition in individuals insensitive to LPS (’low responders‘), up to 80% inhibition in the person with the highest response (’high responder‘) to LPS. Platelets were found to be partially responsible for this difference. Thus, monocytes from high responders cross-combined with platelets from low responders wete much less prone to LPS stimulation than they were in the presence of high responder platelets. Intake of acetylsalicylic acid caused a 50% increment of LPS-induced thromboplastin synthesis, and this effect was mediated by platelets.
点击下载:
PDF
(390KB)
返 回