Cloned Cells Develop Renal Cortical Collecting Tubules
作者:
Makoto Suzuki,
Toshikazu Nakamura,
Masato Ikeda,
Tomoko Hayashi,
Yoshindo Kawaguchi,
Osamu Sakai,
期刊:
Nephron
(Karger Available online 1994)
卷期:
Volume 68,
issue 1
页码: 118-124
ISSN:1660-8151
年代: 1994
DOI:10.1159/000188229
出版商: S. Karger AG
关键词: Hepatocyte growth factor;Morphogenesis;Principal cells;Intercalated cells
数据来源: Karger
摘要:
We investigated the development and morphogenesis of stem cells cloned from cultured transformed collecting duct cells of the rabbit in the presence of hepatocyte growth factor/scatter factor (HGF), vitamin A, laminin and transforming growth factors, HGF and vitamin A induced peanut agglutinin (PNA) positivity in nonprincipal cells (PC) but not in PCs, only HGF induced tubule formation. Although HGF induced a tubule-like arrangement in both cell lines, tubules with PNA-positive cells were observed only in non-PC cells incubated with HGF. Vasopressin increased intracellular free calcium in PNA-negative cells but not in PNA-positive cells. Intracellular pH increased in PNA-positive cells but not in PNA-negative cells when gluconate was substituted for Cl in the buffer. Cloned non-PCs were stem cells that developed PC- and IC-like characteristics and formed collecting ducts when incubated with HGF.
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