The determination of total and unbound midazolam in human plasma. A comparison of high performance liquid chromatography, gas chromatography and gas chromatography/mass spectrometry
作者:
J. X. de Vries,
J. Rudi,
I. Walter‐Sack,
R. Conradi,
期刊:
Biomedical Chromatography
(WILEY Available online 1990)
卷期:
Volume 4,
issue 1
页码: 28-33
ISSN:0269-3879
年代: 1990
DOI:10.1002/bmc.1130040105
出版商: John Wiley&Sons, Ltd.
数据来源: WILEY
摘要:
AbstractMidazolam concentrations in patients' plasma was determined after extraction with high performance liquid chromatography (HPLC), gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). GC was selected for routine plasma assays in terms of selectivity, simplicity, precision, accuracy and sensitivity (0.02 μg/ml); HPLC analysis was less sensitive (0.1 μg/mL) than GC; GC/MS was used for analysis validation. Plasma protein binding of midazolam was determined by GC in patients' plasma after in vitro incubation with midazolam, ultrafiltration and extraction; 5% of the drug was unbound to plasma proteins. Midazolam distribution in lipoprotein fractions separated by ultracentrifugation of plasma obtained from patients on prolonged midazolam treatment was also assayed by G
点击下载:
PDF
(531KB)
返 回