The Use of Osmium-Thiocarbohydrazide for Structural Stabilization and Enhancement of Secondary Electron Images in Scanning Electron Microscopy of Pollen
作者:
WilliamF. Chissoe,
EdwardL. Vezey,
JohnJ. Skvarla,
期刊:
Grana
(Taylor Available online 1995)
卷期:
Volume 34,
issue 5
页码: 317-324
ISSN:0017-3134
年代: 1995
DOI:10.1080/00173139509429065
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Pollen grains stained in a sequence of osmium (O) and thiocarbohydrazide (T) solutions (collectively known as OTOTO) appear structurally stable and undistorted in the scanning electron microscope (SEM), and usually do not require special drying. In fact, OTOTO can be regarded as another special drying method in palynology. This sequential incubation also strikingly increases the electrical conductivity of pollen grains in the SEM. Compared to standard sputter-coating or vacuum evaporative procedures, OTOTO reduces charging and yields secondary electron images with significantly higher resolution.
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