Personal Identification from Human Remains by Mitochondrial DNA Sequencing
作者:
Hiroyoshi Koyama,
Mineo Iwasa,
Susumu Ohtani,
Hiroshi Ohira,
Tsukasa Tsuchimochi,
Yoshitaka Maeno,
Ichiro Isobe,
Tomohiro Matsumoto,
Yoshihiro Yamada,
Masataka Nagao,
期刊:
The American Journal of Forensic Medicine and Pathology
(OVID Available online 2002)
卷期:
Volume 23,
issue 3
页码: 272-276
ISSN:0195-7910
年代: 2002
出版商: OVID
关键词: Personal identification;Mitochondrial DNA;Hypervariable regions;Polymerase chain reaction;Sequencing
数据来源: OVID
摘要:
The authors report four cases in which severely damaged human remains were identified by mitochondrial DNA (mtDNA) sequencing. Degraded DNA was extracted from highly adipoceratous tissues using the phenol-chloroform method and polymerase chain reaction amplified for sequencing of two hypervariable regions, hypervariable region 1 and hypervariable region 2, of mitochondrial DNA. They also sequenced these regions of blood samples that were obtained from the presumptive mother or sister of the human remains. The sequencing results were compared with each other and with the Anderson’s sequence. It was concluded from the sequence data that a lower part of a body in case 1 and some organs in case 2 were from the same woman, and a human head in case 3 and a female body in case 4 were from the relative of a presumptive mother and a sister, respectively.
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