A Time Sharing Fluorometer for the Readout of Intracellular Oxidation‐Reduction States of NADH and Flavoprotein
作者:
B. Chance,
N. Graham,
D. Mayer,
期刊:
Review of Scientific Instruments
(AIP Available online 1971)
卷期:
Volume 42,
issue 7
页码: 951-957
ISSN:0034-6748
年代: 1971
DOI:10.1063/1.1685313
出版商: AIP
数据来源: AIP
摘要:
Cellular fluorochromes, pyridine nucleotides (PN), and flavoproteins (Fp) reflect the redox state of the cytoplasmic space and mitochondria of the intact cells and tissues and afford a direct readout of essential parameters of cell metabolism. A time sharing fluorometer which alternates every 8 msec the overlapping emission and excitation wavelengths of both fluorochromes enables nearly simultaneous recording of the two components. From a spot of several millimeters in diameter an adequate signal‐to‐noise ratio can be obtained at a response time of less than 0.1 sec. In conjunction with the regenerative flow apparatus, the fluorescence of the components can be recorded in times down to 20 msec. Applicability to reaction kinetics in perfused organs and in suspensions of mitochondria as well as to other systems with double fluorescence labels is indicated.
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