Anterior Pituitary Cells from Brattleboro (di/di), Long-Evans and Sprague-Dawley Rats Contain Immunoreactive Arginine Vasopressin
作者:
Stephen J. Lolait,
Alison J. Markwick,
Margaret McNally,
Josephine Abraham,
Ian Smith,
John W. Funder,
期刊:
Neuroendocrinology
(Karger Available online 1986)
卷期:
Volume 43,
issue 5
页码: 577-583
ISSN:0028-3835
年代: 1986
DOI:10.1159/000124584
出版商: S. Karger AG
关键词: Arginine-vasopressin;Anterior pituitary gland;Brattleboro rat;Radioimmunoassay;High-performance liquid chromatography;Immunocytochemistry;Cell culture
数据来源: Karger
摘要:
A radioimmunoassay specific for arginine-vasopressin (AVP) was used to establish the presence of immunoreactive (ir)-AVP in extracts of anterior pituitary glands from Sprague-Dawley (SD) and Long-Evans (LE) rats (3.05 ± 1.0 and 1.66 ±0.9 ng/gland, respectively). Lower levels of ir-AVP (0.56 ±0.26 ng/gland) were detected in anterior pituitary gland extracts from rats with hereditary diabetes insipidus (Brattleboro; di/di). The anterior pituitary gland ir-AVP from each rat strain was further characterized by reverse-phase high-performance liquid chromatography (RP-HPLC). In each case the major peak of immunoreactivity co-migrated with synthetic AVP. By peroxidase-antiperoxidase immunocytochemistry, sparsely distributed cells containing ir-AVP were localized in anterior pituitary sections. Levels of ir-AVP in primary cultures of anterior pituitary cells (from SD rats) increased from 52 ± 5 pg/106 cells at 2 days in vitro to 152 ± 17 pg/106 cells at 3 days; during this period 56 ± 6 pg/ml ir-AVP was secreted into the culture medium. Fewer than 1% of the cells in these cultures were immunostainable for AVP. These data indicate that the anterior pituitary gland of the Brattleboro, Long-Evans and Sprague-Dawley rat contains ir-AVP, and that there is synthesis and secretion of this peptide in primary cultures of anterior pituitary cells in
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