A new model of reticulopodial motility and shape: Evidence for a microtubule‐based motor and an actin skeleton
作者:
Jeffrey L. Travis,
Samuel S. Bowser,
期刊:
Cell Motility and the Cytoskeleton
(WILEY Available online 1986)
卷期:
Volume 6,
issue 1
页码: 2-14
ISSN:0886-1544
年代: 1986
DOI:10.1002/cm.970060103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Allogromia;reticulopods;cytoskeleton;microtubules;actin;saltatory transport;cell shape
数据来源: WILEY
摘要:
AbstractCytoskeletal inhibitors were used as probes to test the involvement of microtubules and actin microfilaments in the development, motility, and shape maintenance of the pseudopodial networks (i e, reticulopodia) of the foraminifers Allogromia sp strain NF and Allogromia laticcllaris. Agents that disassemble cytoplasmic microtubules (cold, colchicine, and nocodazole) arrest all movement but have variable effects on reticulopodial shape. Electron microscopy reveals a granulofibrillar matrix but few, if any, microtubules in these motility‐arrested reticulopods. Allogromiids treated with cytochalasin B or D lose substrate adhesion and undergo dramatic changes in shape and motile behavior, highlighted by the coalescence of reticulopodial cytoplasm into irregularly shaped bodies with chaotic motility. Serial semithick sections of such preparations, viewed by high‐voltage electron microscopy, document a striking rearrangement of microtubules within these cytochalasin‐induced bodies. All aspects of cytochalasin‐altered motility are completely inhibited by colchicine. Actin is present in reticulopodia, as determined by staining with rhodamine‐phalloidin; this staining is not observed in cytochalasin‐treated organisms. These data provide compelling evidence that microtubules are required for reticulopodial motility. An actin‐based cytoskeleton is thought to play a role in maintaining shape, mediating pseudopod/substrate adhesion, and coordinating the various microtubule‐depe
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