MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OFNEISSERIA GONORRHOEAE
作者:
REIDUNN B. Aalen,
WENCHE BLIX Gundersen,
期刊:
Acta Pathologica Microbiologica Scandinavica Series B: Microbiology
(WILEY Available online 1987)
卷期:
Volume 95B,
issue 1‐6
页码: 13-21
ISSN:0108-0180
年代: 1987
DOI:10.1111/j.1699-0463.1987.tb03081.x
出版商: Blackwell Publishing Ltd
关键词: Neisseria gonorrhoeae;plasmids
数据来源: WILEY
摘要:
The plasmid content of one penicillin sensitive and six penicillin resistant strains ofNeisseria gonorrhoeaehas been examined. All strains harbour a small, phenotypically cryptic plasmid of 4.1 kilo base pairs (kb). Four of the penicillin resistant strains carry a beta‐lactamase‐producing plasmid of 7.3 kb. One of these also carries a large plasmid of about 40 kb. The two remaining penicillin resistant strains harbour a smaller beta‐lactamase‐producing plasmid of 5.5 kb. The plasmids have been subjected to digestion with a number of restriction endonucleases, and their restriction maps have been compared. Judging by the maps, the cryptic (C‐) plasmids show great similarities. Except that two of them have 54 additional basepairs (bp), and two have a Hpall site instead of a Ddel site, no differences were found. The larger beta‐lactamase‐producing (B‐) plasmids have identical maps. The smaller seem to be homologous with the larger, except for a deletion of 1.8 kb. There is no correlation between the variant of C‐plasmid and type of B‐plasmid harboured in the penicillin resistant strains. The evolutionary implications suggested by this fi
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