Activation of murine oocytes with Ca2+ionophore and cycloheximide
作者:
L. J. Hagemann,
F. L. Hillery‐Weinhold,
M. L. Leibfried Rutledge,
N. L. First,
期刊:
Journal of Experimental Zoology
(WILEY Available online 1995)
卷期:
Volume 271,
issue 1
页码: 57-61
ISSN:0022-104X
年代: 1995
DOI:10.1002/jez.1402710107
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA Ca2+ionophore (A23187, 3 μM) and inhibitor of protein synthesis (cycloheximide, 10 μg/ml) were used sequentially as a unique method for activating mouse oocytes in vitro. Brief exposure of oocytes to A23187 followed by 6 hr in cycloheximide resulted in a higher activation rate (93.8%) compared to A23187 or cycloheximide alone (37.7% and 36.5%, respectively) or the two reagents in reverse order (29.8%). The parthenogenones consistently contained a single pronucleus and second polar body, and showed a high degree of developmental potential, as assessed by transfer to recipient females or addition of a male pronucleus followed by transfer to recipients. This method is a useful way of obtaining large numbers of activated haploid mammalian oocytes for further developmental studies. © 1995 Wiley‐Liss,
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