An Easy Way to Enhance Absorbance Detection Sensitivity of Waters Quanta-4000 Capillary Electrophoresis System
作者:
NebojsaM. Djordjevic,
Kieran Ryan,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 2
页码: 201-206
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608005506
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Capillary electrophoresis (CE) has established itself as a powerful and widely utilized technique for routine analytical separations, characterized by short run times and high efficiencies. A major drawback to CE is its low concentration sensitivity. Because total system volume is generally only a few μl, the detector flow cell should have a small volume, which imposes a limit on detection sensitivities. In this study, we report a simple method for creating a region of extended path length for absorbance detection in capillary electrophoresis. Up to a six-fold gain in sensitivity, compared to on-column detection, was accomplished when the sleeve-cell was utilized at the detection point.
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