A growth chamber experiment was conducted to compare ammonium thiosulfate, gypsum and elemental sulfur in the form of Agrisul as sources of sulfur for rapeseed (Brassica napusvar. Regent). Rapeseed supplied with ammonium thiosulfate or gypsum produced significantly higher yields than treatments supplied with elemental sulfur. Powdering and mixing of elemental sulfur, as opposed to banding granules, significantly increased dry matter yield of rapeseed. While not always significant, there was a trend towards higher dry matter yields where gypsum granules were mixed as opposed to banded and where ammonium thiosulfate was placed in a band as opposed to being mixed throughout the soil.