Expression Patterns of the Lipopolysaccharide Receptor CD14, and the FC&ggr; Receptors CD16 and CD64 on Polymorphonuclear Neutrophils: Data from Patients with Severe Bacterial Infections and Lipopolysaccharide-Exposed Cells
作者:
Christof Wagner,
Reinhold Deppisch,
Birgit Denefleh,
Friederike Hug,
Konrad Andrassy,
G. Hänsch,
期刊:
Shock
(OVID Available online 2003)
卷期:
Volume 19,
issue 1
页码: 5-12
ISSN:1073-2322
年代: 2003
出版商: OVID
关键词: Apoptosis;IgG receptor;LPS receptor
数据来源: OVID
摘要:
In polymorphonuclear neutrophils (PMN) CD14, one of the receptors for lipopolysaccharides (LPS) is stored intracellularly as a preformed protein, with only few receptors expressed on the surface. We now report that in patients with severe bacterial infections, CD14 expression is profoundly upregulated, as is CD64 (Fc&ggr;RI), the high-affinity receptor for IgG, whereas CD16 (Fc&ggr;RIII) was partly lost from the surface. To further analyze regulation of these receptors, PMN of healthy donors were exposed to low doses of LPS. By brief exposure (10-120 min) to LPS, CD14 was transferred to the surface in a cytochalasin B-sensitive manner, as were CD16 and CD64. Prolonged culture (up to 48 h) resulted in a further upregulation of CD14, sustained expression of CD64, and profound decline of CD16, yielding a similar pattern of receptor expression as seen in the patients. Subsequent studies revealed that LPS inducedde novosynthesis of CD14: the increase of surface expression could be inhibited by cycloheximide and by interfering with a known LPS-induced signaling event, the translocation of NF&kgr;B. Moreover, an up to 10-fold increase of specific mRNA was seen, as was incorporation into CD14 of35S-methionine. Thede novosynthesis prolonged expression of CD14, whereas the CD16 expression declined, generating a PMN phenotype characteristic for severe infection and indicative of escape from apoptosis of a PMN subpopulation.
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