Kinetic Studies of Formaldehyde Binding in Tissue
作者:
HelanderKerstin G.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 3
页码: 177-179
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106282
出版商: Taylor&Francis
关键词: formaldehyde;fixation;kinetics
数据来源: Taylor
摘要:
Specimens of rabbit liver were fixed for various periods up to 6 days in buffered14C-formaldehyde. Binding of the isotope reached a plateau after fixation for approximately 24 hr; the half-maximal binding level was reached after approximately 100 min. Formaldehyde binding at 37 C was faster than at 25 C, and faster at pH 7.0 than at pH 4.0. During rinsing of the fixed tissue in water for up to 26 days there was a progressive decrease in isotope content to 10–20% of the pre-rinse level, indicating that formaldehyde fixation is a reversible process.
点击下载:
PDF (217KB)
返 回