Pollen Wall Structure Using a New Stripping-Sputtering Device for Scanning Electron Microscopy
作者:
Shengyin Lan,
Zhenxiu Xu,
Tingdong Fu,
WaheebK. Heneen,
期刊:
Grana
(Taylor Available online 1995)
卷期:
Volume 34,
issue 5
页码: 325-331
ISSN:0017-3134
年代: 1995
DOI:10.1080/00173139509429066
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new method has been developed to elucidate pollen wall architecture by the separation of wall layers and the use of scanning electron microscopy (SEM). Separation of wall layers at natural boundaries, breakage across the wall and metal-coating of the specimen have been achieved by controlled ramming of free scattered ions produced by a novel “ion separating and coating – model A” instrument. The stripping treatment reveals interfaces and cross profiles of pollen walls and the sputtering treatment results in metal coating for examination with SEM. An advantage of the method is that it provides intact interfaces that are not eroded or damaged. The application of the method is exemplified by SEM analyses of pollen grains ofGossypium hirsutumL.,Zea maysL.,Sesamum indicumL. andBrassica napusL. var.oleifera. Interfaces between the tectum, column, foot, nexine-2 and intine layers of the pollen wall were all portrayed in G.hirsutumand to a great part in the other species. InG. hirsutum, it was possible to document the attachment point of surface spines, the appearance of individual baculae and the irregular labrum-operculum but regular inner labrum-aperture structure. No tectum was found inS. indicum. In all four species it was not possible to separate the intine from the sporoplast. The numbers of apertures were 20, 1, 10–14 and 3 inG. hirsutum, Z. mays, S. indicumandB. napus, respectively. The dumbell-shaped arrangement of apertures inG. hirsutum, the gear-shaped oblate sporoplast ofS. indicumand the abundance of micropores on the intine ofB. napusare characteristic features.
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