Whole Mounted Pressurized in vitro Model for the Study of Cerebral Arterial Mechanics
作者:
Phillip E. Vinall,
Frederick A. Simeone,
期刊:
Journal of Vascular Research
(Karger Available online 1987)
卷期:
Volume 24,
issue 1-2
页码: 51-62
ISSN:1018-1172
年代: 1987
DOI:10.1159/000158671
出版商: S. Karger AG
关键词: Smooth muscle mechanics;Pressure;Middle cerebral artery;Potassium;Serotonin;Norepinephrine;Histamine
数据来源: Karger
摘要:
A whole mounted model for the study of smooth muscle mechanics is described which uses cylindrical sections of middle cerebral artery (MCA). One- to 2-cm sections of artery were cannulated, pressurized and perfused. Changes in the outer diameter of the artery were detected using an infrared photoelectric diameter gauge. MCA displayed spontaneous rhythmic contractions, which were initiated and sensitive to changing intraluminal pressure. Rhythmic activity was dependent upon the presence of Ca2+ in the bath, but not affected by phentolamine. Increasing pressure significantly reduced high (50, 70 and 125 mM) potassium-induced contractions, but not drug (serotonin, norepinephrine, histamine) induced contractions. The intraluminal surface of the MCA was significantly more sensitive to serotonin than the extraluminal surface. The above contractile responses are discussed in light of the advantages of this model over helical or ring preparations.
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