Identification of bloodmeals of the Scottish biting midge, Culicoides impunctatus, by indirect enzyme‐linked immunosorbent assay (ELISA)
作者:
A. BLACKWELL,
A. J. MORDUE (LUNTZ),
W. MORDUE,
期刊:
Medical and Veterinary Entomology
(WILEY Available online 1994)
卷期:
Volume 8,
issue 1
页码: 20-24
ISSN:0269-283X
年代: 1994
DOI:10.1111/j.1365-2915.1994.tb00378.x
出版商: Blackwell Publishing Ltd
关键词: Culicoides impunctatus;Diptera;Ceratopogonidae;ELISA;bloodmeal identification;host preference;feeding patterns
数据来源: WILEY
摘要:
Abstract.An indirect enzyme‐linked immunosorbent assay (ELISA) has been developed to identify the bloodmeal sources ofCulicoides impunctatusGoetghebuer. Nightly light trapping in Scotland, between April and September 1992, caught a total of 344 blood‐engorged femaleC. impunctatus, all in May, June and July. The insects were stored in 70% ethanol and tested 5–7 months later. Bloodmeal sources were positively identified for 246 (71.5%). Bovine hosts were most common (38.4%), followed by deer (23.0%) and sheep (9.9%). Only one midge had fed on human blood. Relatively freshly fed (<24h), fully engorged females gave the clearest results. The wider applications of the technique to the study ofCulicoideshost preferences is disc
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