We report a novel method to assemble colloidal particles to an ordered structure. Individual DNA molecules folded by a sticky condensing agent can be prefabricated into a linear micrometer‐sized pearling chain under trapping field with a focused IR laser. Using this method, the macromolecules are assembled in a sequential order, and then a complex structure is fabricated by arraying them on a solid substrate. This approach may be useful for the further development of micro‐manufacture using macromolecules as nano‐elements. © 2004 American Institute of Physics