Determination of 2′,3′-Dideoxyinosine in Plasma by High Performance Liquid Chromatography
作者:
Gene Ray,
Evelyn Murrill,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 11
页码: 1815-1838
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708082598
出版商: Taylor & Francis Group
关键词: DDI;anti-AIDS drug;HPLC;plasma
数据来源: Taylor
摘要:
A high performance liquid chromatography analysis method has been developed for the quantitation of 2′,3′-dideoxyinosine (DDI) in plasma. Proteins were precipitated from plasma samples with acetonitrile containing the internal standard, 6-methylaminopurine riboside. The treated samples were evaporated to dryness and reconstituted in mobile phase for the analysis. Separation of the components was achieved on a 5 μm octadecylsilane column with ultraviolet detection at 254 nm. The method was validated at nine concentrations between 0.015 and 150 μg/mL. Using 500 μL of human plasma, the limit of quantitation was 120 ng/mL and the limit of detection was 60 ng/mL. The mean intra-day precision of the method was 1.6%. The mean accuracy of the method was within 2% of the actual values. This method is currently being used for pharmacokinetic studies in the rat.
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