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100‐microsecond‐resolved EXAFS technique for studying protein dynamics

 

作者: H. W. Huang,   W. H. Liu,   T. Y. Teng,   X. F. Wang,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1983)
卷期: Volume 54, issue 11  

页码: 1488-1491

 

ISSN:0034-6748

 

年代: 1983

 

DOI:10.1063/1.1137273

 

出版商: AIP

 

数据来源: AIP

 

摘要:

We have built a microcomputer‐controlled x‐ray absorption spectrometer which is capable of measuring 100‐&mgr;s‐resolved extended x‐ray absorption fine structure (EXAFS) of dynamic systems. The spectrometer integrates the x‐ray absorption signals over a time interval &tgr; at any timetmeasured from the time of sample excitation. A series of absorption data of differenttis measured for a given x‐ray energy. The process is repeated for as many different x‐ray energies as required. The lower limit on &tgr; is determined by the concentration of sample and the intensity of synchrotron radiation. The system was recently tested at Stanford Synchrotron Radiation Laboratory using a carboxymyoglobin sample. We present the raw data here to demonstrate the feasibility of this spectrometer.

 

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