100‐microsecond‐resolved EXAFS technique for studying protein dynamics
作者:
H. W. Huang,
W. H. Liu,
T. Y. Teng,
X. F. Wang,
期刊:
Review of Scientific Instruments
(AIP Available online 1983)
卷期:
Volume 54,
issue 11
页码: 1488-1491
ISSN:0034-6748
年代: 1983
DOI:10.1063/1.1137273
出版商: AIP
数据来源: AIP
摘要:
We have built a microcomputer‐controlled x‐ray absorption spectrometer which is capable of measuring 100‐&mgr;s‐resolved extended x‐ray absorption fine structure (EXAFS) of dynamic systems. The spectrometer integrates the x‐ray absorption signals over a time interval &tgr; at any timetmeasured from the time of sample excitation. A series of absorption data of differenttis measured for a given x‐ray energy. The process is repeated for as many different x‐ray energies as required. The lower limit on &tgr; is determined by the concentration of sample and the intensity of synchrotron radiation. The system was recently tested at Stanford Synchrotron Radiation Laboratory using a carboxymyoglobin sample. We present the raw data here to demonstrate the feasibility of this spectrometer.
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