Transfer Thermodynamics of Protein in Denaturing and Stabilizing Media
作者:
Someswar Chatterjee,
I. Basumallick,
期刊:
Journal of the Chinese Chemical Society
(WILEY Available online 2008)
卷期:
Volume 55,
issue 1
页码: 17-22
ISSN:0009-4536
年代: 2008
DOI:10.1002/jccs.200800005
出版商: WILEY‐VCH Verlag
关键词: Cavity forming free energy;Denaturing solvent;Protein stabilization;RibonucleaseA;Scaled particle theory
数据来源: WILEY
摘要:
AbstractFree energies of transfer (ΔGt) of RibonucleaseA (RNaseA) from water to aqueous solutions of urea (4 M, 6 M and 8 M), a protein denaturing solvent as well as ΔGtof RibonucleaseA, β‐Lactoglobulin, α‐Chymotripsin and ChymotrypsinogenA from water to aqueous glycerol (10%, 20%, 30% and 40%), a protein stabilizing solvent has been dissected into cavity term [ΔGt(cav)] and interaction term [ΔGt(int)]. The interaction free energy includes all types of interactions like hard‐soft, hydrogen bonding, electrostatic, etc. The cavity forming free energies have been calculated using the standard version of scaled particle theory (SPT) with well‐reported SPT parameters. It has been found that transfer free energies of cavity terms ΔGt(cav) for native protein from water to urea‐water and water to aqueous glycerol follow almost opposite trends. This primarily indicates there may be some correlation between cavity creation energies and protein denaturing and stabilizing ability of a solvent. The results are in agreement with those obtained from preferential binding coefficient studie
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