HIV‐induced, HIV‐specificin vitroantibody response by B‐cells from HIV‐seropositive individuals
作者:
Jean-François Delfraissy,
Christine Wallon,
François Boué,
Cécile Goujard,
Françoise Barré-Sinoussi,
Pierre Galanaud,
期刊:
AIDS
(OVID Available online 1992)
卷期:
Volume 6,
issue 1
页码: 55-64
ISSN:0269-9370
年代: 1992
出版商: OVID
关键词: HIV;B-cells;in vitro antibody response;interleukin-6 (IL-6);enzyme-linked immunosorbent assay (ELISA).
数据来源: OVID
摘要:
ObjectiveRecent studies have shown that B-cells from HIV-infected patients can secrete anti-HIV antibodiesin vitroand that they represent 20–40% of immunoglobulin (Ig)-secreting B-cellsin vivo.This study was designed to investigate the precise role of HIV in thisin vitroantibody production.Design and methodsB-cells from HIV-infected patients [asymptomatic, n = 28; symptomatic (AIDS), n = 14], from seronegative adult volunteers (n = 22) and subjects at high risk for HIV infection (n = 15) were culturedin vitroin the presence of pokeweed mitogen,Staphylococcus aureuscowan or HIV, and T-cells or interleukins (IL). Non-specific Ig production and specific anti-HIV antibody (Ab) production were measured by enzyme-linked immunosorbent and Western blot assays.ResultsWe found that HIV induced a specific response in cultured B-cells from seropositive patients, in contrast with cultured B-cells from uninfected normal individuals. The characteristics of the HIV-induced response differed from those of a spontaneous or a mitogen-induced response. Anti-HIV Ab production was optimal on day 8–10, when B-cells were cultured with recombinant IL-2 and recombinant interferon-α in the presence of infectious virus or recombinant gp160 Env protein. The anti-HIV Ab were mainly directed against Env proteins. Interaction of HIV with B-cells involved surface IgG but not CD4 antigen. Autologous CD8+ T-cells had a non-specific inhibitory effect. Both CD5 + and CDS - B-cells produced anti-HIV Ab. No anti-HIV Ab production was observed in B-cells from high-risk HIV-seronegative individuals.ConclusionHIV (infectious virus or gp160) can induce B-cells from infected patients to secrete specific anti-HIV Abin vitro.
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