The influence of coronary venous retroinfusion and a vasoselective calcium antagonist felodipine on the microsphere loss in a porcine model of myocardial ischaemia and reperfusion was studied.Sixteen open‐chest pigs underwent 45 min of myocardial ischaemia induced by occlusion of the left anterior descending coronary artery followed by 4 h of reperfusion. Either felodipine (felo‐retro group, 7 nmol kg‐1,n= 6) or the corresponding amount of vehicle (vehicle‐retro group,n= 5) was infused retrogradely into the coronary veins over 30 min, starting 5 min before reperfusion. In a third group, the same amount of felodipine was administered intravenously (felo‐iv group,n= 5). Myocardial regional blood flow was measured with radiolabelled microspheres (ø= 15 μm) injected before ischaemia to investigate a possible loss during ischaemia.In the felo‐retro group, the apparent blood flow in the ischaemic areas, expressed as a percentage of the corresponding values in the non‐ischaemic areas (%‐flow), were 73±15, 73±11 and 75±19 in the subendocardial, midmyocardial and subepicardial layers, respectively. The corresponding percentage flows were 64±11, 70±11 and 62±9 in the vehicle‐retro group and 75±18, 77±15 and 76±11 in the felo‐iv group. The differences between the groups were not satistically significant.It is concluded that in this open‐chest preparation microsphere loss observed in the ischaemic and reperfused myocardium is not increased by coronary venous retroinfusion or by a concomitantly administered va